Intracellular product recycling in high succinic acid producing yeast at low pH

Intracellular product recycling in high succinic acid producing yeast at low pH

BACKGROUND The metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, the product transport can become bidirectional, i.e. the acid is reentering the cell and is again exported or even catabolized. Here, a quantitative approach for the identifica...

The Bacteroides by-product succinic acid inhibits neutrophil respiratory burst by reducing intracellular pH.

Short-chain fatty acids, particularly succinic acid, are major metabolic by-products of Bacteroides species. To determine their role as potential virulence factors in infections containing Bacteroides species, short-chain fatty acids were examined for their effect on the neutrophil respiratory burst. Succinate (30 to 50 mM) irreversibly impaired superoxide and hydrogen peroxide production in re...

The intracellular concentration of succinic acid in photosynthetic bacteria.

Reduction of dehydroascorbic acid at low pH.

Ascorbic acid and dehydroascorbic acid are unstable in aqueous solution in the presence of copper and iron ions, causing problems in the routine analysis of vitamin C. Their stability can be improved by lowering the pH below 2, preferably with metaphosphoric acid. Dehydroascorbic acid, an oxidised form of vitamin C, gives a relatively low response on the majority of chromatographic detectors, a...

5i. the Formation of Succinic- Acid in Yeast

PASTEUR [1860] was the first to observe succinic acid among the substances formed during alcoholic fermentation. In his experiments the yield of succinic acid was 0-5-0 7 % of the fermented sugar. Ehrlich [1909] found that fermenting yeast is capable of converting glutamic acid into succinic acid and advanced the view that all the succinic acid formed during fermentation is derived from glutami...